新黴素ELISA快速檢測試劑盒(檢組織/乳製品，靈敏度0.5ppb，一步法)

                         新黴素ELISA快速檢測試劑盒

一、原理

本試劑盒采用間接競爭(zheng) ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物新黴素將和微孔條上預包被的偶聯抗原競爭(zheng) 抗新黴素抗體(ti) ，加入酶標記物後，用TMB底物顯色，樣本吸光值與(yu) 其所含殘留物新黴素的含量成負相關(guan) ，與(yu) 標準曲線比較即可得出相應殘留物新黴素的含量。

二、試劑盒特性

u  試劑盒靈敏度：   0.5ppb

u  孵育溫度：       25℃

u  孵育時間：       60min～15min

u  樣本檢測下限

組織（雞肉/肝、豬肉/肝、蝦、魚 ·········  10ppb

蜂    蜜 ·········································  10ppb

牛奶、奶粉·······································  10ppb

u  交叉反應率

新黴素  ·········································   100%

鏈黴素  ·········································  ﹤0.1%

慶大黴素  ······································  ﹤0.1%

雙氫鏈黴素  ···································  ﹤0.1%

卡那黴素  ······································  ﹤0.1%

妥布黴素  ······································  ﹤0.1%

紫蘇黴素  ······································  ﹤0.1%

u  樣本回收率

組織（雞/豬肉、蜂蜜、蝦、魚）··········  85±19%

雞肝、豬肝  ···································  70±17%

牛奶、奶粉  ···································  85±16%

三、試劑盒組成

四、樣品前處理及檢測步驟

    詳見產(chan) 品說明書(shu) 。

五、儲(chu) 藏條件和保質期

1、 儲(chu) 藏條件：試劑盒於(yu) 2～8℃保存，不要冷凍。

2、 保 質 期：該產(chan) 品有效期為(wei) 1年，生產(chan) 日期見包裝盒。